Journal: bioRxiv
Article Title: Orbitofrontal Cortex Modulates Auditory Cortical Sensitivity and Sound Perception
doi: 10.1101/2023.12.18.570797
Figure Lengend Snippet: (A) Extracellular recordings were made from 64-channel electrode arrays chronically inserted into the left OFC. Sample recordings illustrate filtered voltage traces from four adjacent channels capturing activity from the same unit. (B) Histological images were used to confirm electrode placement by visualizing fluorescently labeled tracks (green) in the OFC. (C) OFC activity was recorded during task engagement and during passive sound exposure sessions just before (‘passive-pre’) and just after (‘passive-post’) the task. The same units were recorded across all three sessions. (D) Peristimulus time-histograms of two representative OFC neurons illustrate the effect of task engagement on non-AM firing rates. The ‘task-enhanced’ neuron (blue) exhibited a significant increase in firing during the task, while the ‘task-suppressed’ neuron (orange) exhibited a significant decrease. Mean ± standard deviation waveforms for each unit are shown on the left. (E) The magnitude of context modulation for each unit was calculated as the difference between the non-AM firing rate during the task and the non-AM firing rate during the passive-pre session z-scored over trials. Modulation values are presented for all units sorted in increasing order. Gray bars represent units without significant contextual modulation. (F) Absolute modulation magnitudes were similar between task-enhanced and - suppressed units. (G) Task-suppressed units exhibited reductions in non-AM firing that persisted into the passive-post period. The activity of task-enhanced units, on the other hand, returned to passive-pre levels after the task ended. ***p<0.001
Article Snippet: Raw signals were sent to an RS4 data streamer (TDT) and stored online. (II) Signals were preamplified and digitized at 30 kHz by a 64-channel RHD headstage (Intan Technologies) connected to a slip-ring commutator (Taidacent; purchased from Amazon) and integrated with behavioral timestamps using an RHD recording controller (Intan) and OpenEphys software ( ).
Techniques: Activity Assay, Labeling, Standard Deviation
